Table 1. Characteristics of peptides selected from the constant region of antibodies.
Peptide | Amino acid sequence | Ig class | Proteases involved in cleavage | %a | Hydrophobicityb | pI | M.M. (Da) | Net charge |
H4L | HEAL | IgG, IgM, IgAc | - | - | +-00 | 5.12 | 468.49 | − |
N10K | NQVSLTCLVK | IgG1c | Tryp+Tryp | 81,4 | **0*0**00+ | 8.23 | 1104.33 | + |
T11F | TCRVDHRGLTF | IgMd | Chym+Chym | 63,5 | **+0-++00*0 | 8.27 | 1304.51 | + |
cleavage probability;
0: hydrophobic, *: polar, +, and −: positively charged and negatively charged residues;
conserved amino acids in human Ig and different organisms;
conserved amino acids only in human Ig;
pI, isoelectric point; M.M., molecular mass (dalton); Tryp, trypsin; Chym, chymotrypsin.