Table 3. In vitro comparative fungicidal activity of N10K alanine-substituted derivatives against Candida albicans SC5314.
| Anti-Candida activity | ||||
| asd1 | M.M. (Da) | %* | EC50 **(95% confidence intervals) [mol/liter]×10−6 | EC50 asd/EC50 N10K |
| N1A | 1061.30 | 100 | 0.587 (0.586–0.588)§ | 0.58 |
| Q2A | 1047.28 | 100 | 2.305 (2.260–2.350)§ | 2.30 |
| V3A | 1076.28 | 100 | 5.929 (5.064–6.941)§ | 5.91 |
| S4A | 1088.33 | 100 | 2.391 (2.076–2.755)§ | 2.38 |
| L5A | 1062.25 | 100 | 0.885 (0.841–0.931)§ | 0.88 |
| T6A | 1074.30 | 100 | 4.878 (4.364–5.453)§ | 4.86 |
| C7A | 1072.27 | 73.41 | 54.874 (26.243–114.710)§ | 54.66 |
| L8A | 1062.25 | 100 | 0.744 (0.732–0.755)§ | 0.74 |
| V9A | 1076.28 | 100 | 1.776 (1.742–1.810)§ | 1.77 |
| K10A | 1047.23 | 0 | n.d. | n.d. |
1
asd, alanine-substituted derivative; M.M., molecular mass (dalton);
*
% inhibitory activity at 100 µg/ml in comparison to the control growth (water);
**
EC50, half maximal effective concentration;
§
Anti-Candida activity significantly different from that of N10K peptide as assessed by t test (P<0.001).
n.d., not determined.