Fig. 1.

Myristoylation alters protein localization and environment of residues in receptor-binding regions. (A) SDS-PAGE gels of indicated Gα proteins were reconstituted with Gβγ prior to binding to ROS in the dark, in the light, and after light activation followed by addition of GTPγS, as described in methods. Supernatant from dark sample, DS; pellet fraction in dark, DP; light supernatant, LS; light pellet, LP; supernatant, light + GTPγS, GS; pellet, light + GTPγS, GP. (B) Quantitation of A from densitometry of gels. Each bar (red, myr; blue, unmyr) is the average of three independent experiments; results are mean ± SEM.