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. 2012 Feb 28;4(1):10–27.

Figure 9.

Figure 9

Influence of different proteases on Caco-2 mono-layer integrity [modified according to Kolac et al., 1996 in reference 60]. (A) Influence of proteases on the Trans-Epithelial Electrical Resistance (TEER) of confluent Caco-2 mono-layer (o) control with fluorescein; (•) 17.75 F.I.P.-units/well trypsin in KRB; (▴) 1.23 F.I.P.-units/well bromelain in KRB; (▪) 1.75 F.I.P.-units/well papain in KRB; (♦) 745 F.I.P.-units/ well chymotrypsin in KRB. (B) Influence on transport of the transport marker substrate fluorescein (legend as before). FITC = Fluoroisothiocyanate; DMEM = Dulbecco's Modified Eagle Medium; kDA = Kilo-Dalton; KRB = Krebs Ringer buffer.