Figure 2. Microinjection of dynamin-DN into the vlPAG reduces DERM-A594 antinociception and internalization.
Rats were microinjected with the dynamin inhibitory peptide (dyn-DN) or the scrambled peptide (dyn-scr) as a control prior to microinjection of DERM-A594 into the vlPAG. A: Pretreatment with dynamin-DN reduced the antinociceptive effect of microinjecting DERM-A594 into the vlPAG compared to pretreatment with the scrambled peptide. B: The number of DERM-A594 labeled cells was significantly decreased in rats pretreated with dynamin-DN compared to scrambled-dynamin pretreated animals. C, Panel 1, Labeling for DERM-A594 from a representative animal pretreated with scrambled-dynamin (left) compared to a representative animal pretreated with dynamin-DN (right).