Table 1.
Grading scheme for assessment of chondrocyte viability in the mouse knee joint from nitroblue tetrazolium (NBT) staining in viable cells. Maximum possible site score of 5 for the medial and lateral femoral condyles and tibial plateau. Maximum possible total joint score of 20 summed from site scores for each joint.
Grade | Description |
---|---|
0 | ≥ 95% viable cells in all of plateau or condyle |
1 | cell death in < ½ plateau or condyle, < ½ depth |
2 | cell death in < ½ plateau or condyle, ≥ ½ depth |
3 | cell death in ≥ ½ plateau or condyle, < ½ depth |
4 | cell death in ≥ ½ plateau or condyle, ≥ ½ depth |
5 | < 10% viable cells in all of plateau or condyle |