Table 1.
Transcriptional alterations in Smad4 mutant ureters
| Target | Definition | Array Fold Change | qPCR Fold Change |
|---|---|---|---|
| Upregulated in mutants | |||
| muscle-related genes | |||
| Myh3 | Myosin, heavy polypeptide 3 | 4.896 | 3.603 |
| Cdh15 | Cadherin 15 | 3.584 | n.d. |
| Tpm2 | Tropomyosin 2, β | 3.022 | 1.827 |
| Actn2 | Actinin α2 | 2.577 | 1.04 |
| Chodl | Chondrolectin | 2.150 | n.d. |
| Atp2a1 | ATPase, Ca2+ transp, fast twitch 1 | 2.140 | n.d. |
| Myo18b | Myosin XVIIIb | 2.072 | 1.69 |
| Myom2 | Myomesin 2 | 2.013 | 5.45 |
| extracellular matrix-related genes | |||
| Col7a1 | Collagen, type VII, α1 | 3.004 | 2.90 |
| Cntnap4 | Contactin associated protein-like 4 | 2.672 | 1.661 |
| Lama1 | Laminin, α1 | 2.419 | 3.115 |
| Col20a1 | Collagen, type XX, α1 | 2.152 | 1.015 |
| Col6a2 | Procollagen, type VI, α2 | 2.079 | 1.479 |
| kidney/ureter-related genes | |||
| Etv4 | Ets variant gene 4 | 2.550 | n.d. |
| Robo1 | Roundabout homolog 1 | 2.133 | 1.74 |
| Kif26a | Kinesin family member 26A | 2.092 | 1.096 |
| other important genes | |||
| BMP4 | Bone morphogenetic protein 4 | 2.290 | 2.005 |
| OSR2 | Odd-skipped related 2 | 5.726 | 8.706 |
| Downregulated in mutants | |||
| muscle-related genes | |||
| Hopx | HOP homeobox | −2.049 | −1.69 |
| Casq1 | Calsequestrin 1 | −2.073 | −3.05 |
| Actg2 | Actin, γ2, smooth muscle | −2.177 | −4.110 |
| Myom1 | Myomesin 1 | −2.199 | −8.801 |
| Lin7a | Lin-7 homolog A | −3.726 | n.d. |
| extracellular matrix-related genes | |||
| Tspan2 | Tetraspanin 2 | −2.038 | −1.59 |
| Cav2 | Caveolin 2 | −2.108 | −1.53 |
| kidney/ureter-related genes | |||
| Lmcd1 | LIM and cysteine-rich domains 1 | −2.308 | n.d. |
| Cyp2e1 | Cytochrome P450, 2e, polypeptide | −2.389 | n.d. |
| Id2 | Inhibitor of DNA binding 2 | −2.594 | −3.049 |
| other important genes | |||
| STAC | Src homology3 and cysteine-rich domain | −3.189 | −1.721 |
| PCP4L1 | Purkinje cell protein 4-like 1 | −3.363 | −3.277 |
qPCR, quantitative PCR; n.d., not determined.