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. 2012 Apr;18(4):626–639. doi: 10.1261/rna.030965.111

FIGURE 2.

FIGURE 2.

Validation of novel is-ncRNAs. (A) RT-PCR validation of novel is-ncRNAs. Of 12 randomly chosen is-ncRNAs, all were verified by RT-PCR. Each is-ncRNA is represented by three adjacent lanes, from left to right: (G) genomic DNA PCR, positive control); (+) DNase-treated RNA RT-PCR; (−) DNase-treated RNA PCR (no RT; negative control). (B) Six is-ncRNAs verified by Northern blot. Arrows indicate the size of the is-ncRNA as obtained from the sequencing data.