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. 2012 Apr;18(4):704–719. doi: 10.1261/rna.030742.111

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FIGURE 8. — Hnrpab isoforms are predominantly nuclear in developing neurons. Primary hippocampal neurons from Hnrpab^+/− (A,B) and Hnrpab^−/− (B,D) E18 mouse brains were dissociated and plated, then maintained in culture for 5 d before infection with recombinant LVPs to express Flag epitope-tagged either Hnrpab1 (A,B) or Hnrpab2 (C,D). These were fixed and processed for immunostaining 3 d later (8DIV), using anti-Flag epitope antibodies and DAPI for the nucleus. Pseudo-colored Flag immunofluorescence (orange) merged with DAPI (blue) and DIC images (gray) (panels labeled 1) are shown next to the Flag immunofluorescence images alone (panels labeled 2). Scale bars represent 20 μm.