Skip to main content
. 2012 Apr;18(4):720–728. doi: 10.1261/rna.030874.111

FIGURE 1.

FIGURE 1.

Typical results of the primer extension analysis of RNase P/MRP RNA crosslinked to specific proteins. (A) RNase MRP RNA crosslinked to Pop1; (B) RNase P RNA crosslinked to Pop7; (C) RNase MRP RNA crosslinked to Pop7; (D) RNase P RNA crosslinked to Pop4. (Lanes 1,2,12,13,23–26,37,47,48) Sequencing markers; (lanes 3,14,27,38) primer extension reactions for deproteinated RNAs (controls); (lanes 11,22,35,46) primer extension reactions for UV-treated (1280 mJ/cm2) deproteinated RNAs (controls); (lanes 4–10,15–21,28–34,39–45) primer extension reactions for UV-crosslinked RNAs, the UV dose increasing from 0 to 640 mJ/cm2. The arrows and boxed regions indicate the locations of the RNA–protein crosslinks.