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. 2012 Apr;18(4):720–728. doi: 10.1261/rna.030874.111

FIGURE 4.

FIGURE 4.

Isolation of His-tagged proteins from RNase P/MRP holoenzymes. (Lanes 1,2) Protein Pop1 (yeast strain EK-Pop1NHis); (lanes 3,4) protein Pop3 (yeast strain EK-Pop3); (lanes 5,6) protein Pop4 (yeast strain EK-Pop4); (lanes 7,8) protein Pop5 (yeast strain EK-Pop5); (lanes 9,10) protein Pop6 (yeast strain EK-Pop6); (lanes 11,12) protein Pop7 (yeast strain EK-Pop7); (lanes 13,14) protein Pop8 (yeast strain EK-Pop8); (lanes 15,16) protein Rmp1 (yeast strain EK-Rmp1); (lanes 17,18) protein Rpp1 (yeast strain EK-Rpp1); (lanes 19,20) protein Snm1 (yeast strain EK-Snm1); (lanes 21,22) protein Rpr2 (yeast strain EK-Rpr2). Odd lanes are isolated mixtures of RNase P/MRP holoenzymes; even lanes are pull-downs of tagged proteins (marked by black stars). Silver-stained SDS-polyacrylamide gels.