FIGURE 7.

Reduced expression of OASL, IFI44, and SPINK4 in cells where the expression of MALAT-1, RNPS1, SRm160, or IBP160 was repressed by RNAi. (A) Total RNAs were prepared from cells transfected with siRNAs against MALAT-1 and then analyzed by qRT–PCR. Using two different siRNAs against MALAT-1 minimizes the possible off-target effects, causing apparent reductions of transcript levels. Each value was normalized to the expression level of glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Error bars indicate the standard deviation (SD) obtained from three independent experiments. (B) Pre-mRNA levels of the indicated transcripts were measured by qRT–PCR. The primer set for OASL pre-mRNA and the primer set for IFI 44 pre-mRNA amplified the cDNA sequence in the exon 1 and intron 1 junction of each pre-mRNA. The primer set for SPINK4 pre-mRNA amplified the cDNA sequence in the junction between exon 3 and intron 3 of SPINK4. (C) The indicated transcripts were quantified by qRT–PCR analysis using total RNAs prepared from cells transfected with the indicated siRNAs. Each value was normalized to the expression level of GAPDH. Error bars represent the SD obtained from three independent experiments.