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. 2012 Apr;18(4):844–855. doi: 10.1261/rna.029413.111

FIGURE 4.

FIGURE 4.

PNPase, but not RNase E or RNase III, degrades the Hfq-free MicA* RNA. (A) Northern blot detection of MicA RNA in Hfq− cells harboring or not harboring the rne-1 allele. Stationary-phase cultures were treated at 44°C for inactivation of the thermosensitive RNase E (as mentioned before). MicA RNA stability was analyzed by Northern blot with a specific riboprobe. (B) Northern blot analysis of MicA in Hfq− cells deficient in RNase E or PNPase. The double hfq rne-1 mutant was grown at 30°C until stationary phase and then incubated at 44°C to inactivate RNase E. For comparison, the hfq and hfq pnp were treated in the same conditions. (C) Northern blot detection of MicA in stationary-phase cultures of Hfq− cells harboring or not harboring RNase III (rnc), respectively. A loading control corresponding to a nonspecific band that cross-reacted with MicA probe is shown in below. (D) Comparison of MicA* RNA steady-levels in Hfq− stationary-phase cells deficient in RNase III or PNPase grown at 37°C.