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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Mol Cancer Res. 2012 Jan 24;10(3):378–391. doi: 10.1158/1541-7786.MCR-11-0314

Figure 6. The N-terminus of LEDGF/p75 interacts with MeCP2.

Figure 6

(A) Diagram of LEDGF/p75 deletion constructs used to map interaction regions. (B) Flag-MeCP2 binds to eGFP-LEDGF/p75 but not to eGFP-tagged truncated LEDGF/p75 constructs. 293T cells ectopically overexpressing the tagged proteins labeled in the blot were immunoprecipitated with GFP antibody and visualized by immunoblotting with antibodies to GFP and Flag. (C) Flag-MeCP2 binds to eGFP-tagged LEDGF/p75 and LEDGF/p52 but not to truncated constructs. Proteins ectopically overexpressed in U2OS cells were immunoprecipitated with Flag antibody and visualized with both anti-GFP and anti-Flag antibodies. *Denotes degradation product of LEDGF/p75. (D) Recombinant Flag-LEDGF/p75, Flag-PWWP-CR1 (aa 1–141) and Flag-PWWP (aa 1–101) were incubated with U2OS cell lysate. Proteins pulled down with anti-Flag affinity matrix were detected by immunoblotting. Endogenous MeCP2 in the cell lysate was pulled down by Flag-LEDGF/p75 and Flag-PWWP-CR1. Absence of recombinant proteins (U2OS only) served as negative control.