Figure 4.

Overlaid display of the two images 5μm apart for best variant of each method. (a): Ground truth (aligned images). (b): Randomly transformed images. (c): PCA alignment with nuclei centers. (d): MI with PCA alignment (nuclei centers) and images filtered at optimal σ_f. (e): KDE with PCA alignment (nuclei centers) and optimal σ_k. Note the increasing improvement in cell alignment (yellow vertical arrow pointing to a single cell) from (c) to (e). KDE initialized with PCA gives the best results in (e). Also note the overlap between cyan and red arrows in the center indicating progressive improvement in alignment