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. 2011 Dec 20;137(3):279–291. doi: 10.1007/s00418-011-0899-7

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© The Author(s) 2011

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Fig. 7 — Double immunofluorescence staining with polyclonal antibody directed against MC4R (red) and F4/80 (green) followed by fluorescent immunodetection in sections of mouse liver during APR. Upper control liver, lower 6 h after TO treatment. Inset shows higher magnification. White arrow indicates the hepatocytes positive for MC4R and white arrowhead indicates the Kupffer cells positive for MC4R and F4/80. Results show the representative picture of three animals and six slides (original magnification, ×200, scale bar 20 μm)