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Effect of D-4F on lipid oxidation and deposition in Ldlr−/− mice. (A) Dichlorofluorescein (DCF) assay for lipid oxidation products in chow, HFD (PBS and PTH), and D-4F (PTH/D-4F and D-4F) groups. (B) Oil red O staining for lipids (red) in spleen, kidney, and femoral bone cryosections counterstained with hematoxylin. Scale bar =50 μm. *p <.05.
