Skip to main content
. 2010 Dec 15;26(6):1230–1241. doi: 10.1002/jbmr.314

Fig. 2.

Fig. 2

rhNell-1 induces Nfatc2 protein synthesis in ATDC5 cells. (A) Western blot of total lysates of ATDC5 cells treated with 800 ng/mL of rhNell-1 for 0 to 10 hours. β-actin was included as a protein loading control. (B) Quantitative plot of Nfatc2 protein level normalized to β-actin using Quantity One software. Data represent the mean ± SD from tests of samples in triplicate. *p <.05; **p <.01 versus control (0 hour). (C) Subcellular localization of Nfatc2 protein in ATDC5 cells with or without 800 ng/mL of rhNell-1 treatment for 3 hours. Nfatc2 protein was stained with anti-Nfatc2 antibody (green), and nuclei were counterstained with Hoechst 33342 (blue). Representative image from one of three independent experiments. Original magnification ×200.