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. 2012 Feb 14;5:93. doi: 10.1186/1756-0500-5-93

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Copyright ©2012 Zhao et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effect of the isolation procedure on the surface expression of CD14 on neutrophils. Whole blood or isolated neutrophil was stained with CD14 mAb. Blood was anticoagulated with HEPARIN. Using flow cytometry, the percentage of PMN expressing mCD14 (3A) and the mean channel fluorescence intensity (MFI) (3B) was measured. Results for each treatment are the mean from 5 cows. Treatment means with different letters are significantly different (p < 0.05) using Scheffe's multiple comparison tests.