Figure 1.

Typical TIC of the VOCs from the culture medium samples of WI-38 VA13 cells (A), OUS-11 cells (B) and A549 cells (C) after 1-week incubation. The TICs were obtained from analysis of the samples (200 μL) by HS-SPME (DVB/CAR/PDMS, 50/30 μm, 2 cm) and GC-TOF MS. The extraction temperature was 45°C and the time was 50 min. Desorption was performed at 240°C for 10 min. The injection was pulsed splitless (closed 3 min) with a 0.75 mm liner. Temperature programming consisted of an initial temperature 40°C for 5 min, followed by 3°C/min to 240°C with a 5 min hold at this final temperature. The other GC-MS conditions are described in the Material and Methods.