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. 2012 Mar 26;7(3):e34169. doi: 10.1371/journal.pone.0034169

Figure 3. HBV envelope proteins are degraded in the presence of EDEM1, in a dose dependent manner.

Figure 3

(A) HEK293T cells were transfected with pCiS in the presence or absence of pCMVEDEM1. Cell lysates were split in two and equal amounts of proteins were subjected to SDS-PAGE under reducing conditions, followed by Western blotting with anti-EDEM (upper panel) or anti-S (lower panel) Abs. Controls, mock-transfected cells were also included. Calnexin (Cnx) expression was used a total protein, gel loading control. (B) The relative band intensities were quantified and compared to control (considered 100%), using the “Quantity One” software from BioRad.