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. Author manuscript; available in PMC: 2013 Jun 5.
Published in final edited form as: Virology. 2012 Mar 9;427(2):127–134. doi: 10.1016/j.virol.2012.02.010

Figure 2.

Figure 2

Growth analysis and stability of DENV-1 T329A neutralization escape variant. A–B. Multi-step growth curves of wild type or T329A West-Pac-74 DENV-1 in (A) BHK21-15 or (B) C6/36 cells. Cells were infected at an MOI of 0.01 and supernatants were harvested at 24-hour intervals for titration by plaque assay. The data are the average of three independent experiments performed in duplicate and asterisks indicate differences that are statistically significant. C–D. Supernatants were harvested at 96 hours from wild type or T329A DENV-1 infected (C) BHK21-15 or (D) C6/36 cells and used for PRNT analysis to assess stability of the escape mutation in cell culture. The data are representative of two independent experiments performed in duplicate on BHK21-15 cells.