Table 1.
Infection and reversion of DENV-1 T329A in Aedes aegypti
| 0 to 1 hour post infection |
14 days post infection |
14 days post infection |
|
|---|---|---|---|
| Virus | Number positive (%) |
Number infected (%) |
Number revertants (%) |
| T329A | 10/10 (100) | 15/69 (22) | 1/15 (7) |
| Wild type | 10/10 (100) | 0/47 (0) | ND |
| P-value | NS | 0.0003 | ND |
Aedes aegypti mosquitoes were fed orally wild type (3.1 × 106 PFU/ml) or T329A (2.6 × 106 PFU/ml) DENV-1 in a 1:1 blood to virus ratio. Engorged mosquitoes were harvested immediately (within one hour) after feeding and screened for presence of DENV RNA or held for a 14-day extrinsic incubation period, after which bodies were harvested for viral RNA sequencing as described in the Methods. The results are pooled from independent experiments, and the proportions infected were compared by Fisher’s exact test. ND indicates not determined, as mosquitoes were not infected with the wild type parent DENV-1 West-Pac-74 strain under these conditions. NS indicates no significant difference.