Fig. 2.
MDA concentrations in culture medium and HO-1 expression in cell lysates of HUVECs carrying three genotypes under different levels of OS. HUVECs were freshly isolated from umbilical cord veins (S/S [n = 7], S/L [n = 13] or L/L [n = 5], respectively), and exposed to 0, 25, 50µmol/L of H2O2 for 3 hours. (a) Values are means ± S.D. *Ptrend < 0.001 (b) Values are means ± S.D. Under 25µmol/L H2O2, HUVECs carrying S/S had significantly higher HO-1 expression levels than L/L and S/L(*P < 0.05), whereas there was no significant difference between S/L and S/S. Under 50µmol/L H2O2, HUVECs carrying S/L had significantly higher HO-1 expression levels than L/L (*P < 0.05); HUVECs carrying S/S had significantly higher HO-1 expression levels than S/L (*P < 0.05) and L/L (**P < 0.001)