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. Author manuscript; available in PMC: 2013 May 15.
Published in final edited form as: Mol Cell Endocrinol. 2012 Feb 10;355(1):114–120. doi: 10.1016/j.mce.2012.01.027

Figure 4.

Figure 4

High fat diet induces TNFα and C/EBPβ expression in mammary gland of overweight female Aromhum mice. After the 3-month diet treatments, mammary glands of female Aromhum mice were removed and subjected to (A) RNA extraction and real-time RT-PCR to quantify mouse TNFα, C/EBPβ, JunB, and JunD mRNA levels, and (B) tissue protein extract preparation and immunoblotting with anti-C/EBPβ and anti-actin antibodies. Normal chow group, n=4; high fat diet group, n=5. **, P<0.01 (paired t test).