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. Author manuscript; available in PMC: 2013 Apr 3.
Published in final edited form as: J Proteomics. 2012 Jan 17;75(7):2153–2159. doi: 10.1016/j.jprot.2012.01.012

Figure 1.

Figure 1

Immunodetection of cortactin binding partners in M2 agarose eluates. SDS PAGE of M2 agarose eluates. Eluates were electrophoresed in 8% PAGE-SDS gel, blotted and stained with Ponceau followed by incubation with a mixture of anti-Flag, anti-dynamin and anti-ARP3 antibodies followed by chemiluminescent detection as described in the text. Lane 1 : Flag-FAK (FF) expressing cells. Lane 2: Flag-cortactin (F-cort). Lane 3: Flag-cortactin DD mutant. Lane 4: Flag-cortactin WK mutant.