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. Author manuscript; available in PMC: 2013 Apr 3.
Published in final edited form as: J Proteomics. 2012 Jan 17;75(7):2153–2159. doi: 10.1016/j.jprot.2012.01.012

Figure 3.

Figure 3

A) Distribution of EGFP cortactin in diving HEK 293 cells. Two examples of EGFP-cortactin expressing HEK 293 cells 6 h after release from nocodazole driven cell cycle arrest. Arrows point to zones of marked intensity of cortactin statining in the equatorial zone of the dividing pairs of cells. B) Co-immmunoprecipitation of endogenous cortactin and RCC2/TD60 from lysates of nocodazole synchronized Hela cells. Endogenous cortactin was immunoprecipitated with monocloncal antibody 4F11 followed by western blot analysis with anti- RCC2/TD60 polyclonal antibody as described in the text. Lanes 1 and 2 corresponds to a control non-related monoclonal antibody and anti-cortactin antibody 4F11, respectively. In Lane 3 is seen an aliquot of the total cell lysate.