Table I.
Distribution of exclusively heavy or light labeled peptides eluted from M2-agarose beads from SILAC experiments
| Exclusively “Light” | Exclusively “Heavy” | ||||
|---|---|---|---|---|---|
| Protein name | Accession number | # Peptides | Protein name | Accession number | # Peptides |
| Keratin, type II cytoskeletal 1 | IPI00220327 | 27 | 60S ribosomal protein L30 | IPI00219156 | 4 |
| Keratin, type I cytoskeletal 9 | IPI00019359 | 21 | Isoform Long of Splicing factor, proline- and glutamine-rich | IPI00010740 | 5 |
| Keratin, type II cytoskeletal 2 epidermal | IPI00021304 | 15 | 60S ribosomal protein L6 | IPI00790342 | 3 |
| Keratin, type I cytoskeletal 14 | IPI00384444 | 6 | 60S ribosomal protein L7a | IPI00299573 | 4 |
| Isoform 1 of Gelsolin | IPI00026314 | 10 | Fructose-bisphosphate aldolase A | IPI00465439 | 3 |
| Keratin, type I cytoskeletal 10 | IPI00009865 | 19 | Nucleosome assembly protein 1-like 1 | IPI00023860 | 3 |
| Keratin, type II cytoskeletal 5 | IPI00009867 | 3 | Isoform 1 of Eukaryotic translation initiation factor 3 subunit B | IPI00396370 | 3 |
| D-3-phosphoglycerate dehydrogenase | IPI00011200 | 3 | 40S ribosomal protein S25 | IPI00012750 | 3 |
| Keratin, type II cytoskeletal 1 | IPI00220327 | 18 | 60 kDa heat shock protein, mitochondrial | IPI00784154 | 6 |
| Keratin, type I cytoskeletal 9 | IPI00019359 | 16 | cDNA FLJ59211, highly similar to Glucosidase 2 subunit beta | IPI00026154 | 3 |
| Probable serine carboxypeptidase CPVL | IPI00301395 | 3 | Isoform 1 of Dynamin-2 | IPI00033022 | 5 |
| Isoform 1 of Transcription intermediary factor 1-beta | IPI00438229 | 4 | 40S ribosomal protein S16 | IPI00221092 | 5 |
| Isoform 3 of Spectrin alpha chain, brain | IPI00843765 | 5 | 60S ribosomal protein L23a | IPI00021266 | 3 |
| Keratin, type I cytoskeletal 10 | IPI00009865 | 10 | Actin-related protein 2 | IPI00005159 | 6 |
| Isoform Short of Heterogeneous nuclear ribonucleoprotein U | IPI00479217 | 4 | Serine/threonine-protein phosphatase 2A catalytic subunit alpha | IPI00008380 | 4 |
| Heterogeneous nuclear ribonucleoprotein H | IPI00013881 | 4 | Actin-related protein 2/3 complex subunit 1A | IPI00333068 | 7 |
| Profilin-1 | IPI00216691 | 4 | Protein RCC2 | IPI00465044 | 4 |
| Myristoylated alanine-rich C-kinase substrate | IPI00219301 | 4 | Actin-related protein 2/3 complex subunit 3 | IPI00005162 | 3 |
| 60S acidic ribosomal protein P2 | IPI00008529 | 4 | Actin-related protein 2/3 complex subunit 4 | IPI00554811 | 6 |
| Vimentin | IPI00418471 | 6 | |||
| T-complex protein 1 subunit alpha | IPI00290566 | 3 | |||
| Importin subunit beta-1 | IPI00001639 | 4 | |||
| Isoform 1 of E3 ubiquitin-protein ligase TRIM21 | IPI00018971 | 3 | |||
| Peptidyl-prolyl cis-trans isomerase FKBP10 | IPI00303300 | 3 | |||
| Isoform 1 of Myosin-9 | IPI00019502 | 10 | |||
|
| |||||
| 25 proteins identified from 209 peptides | 19 proteins indentified from 80 peptides | ||||