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. Author manuscript; available in PMC: 2013 Mar 23.
Published in final edited form as: Immunity. 2012 Feb 16;36(3):401–414. doi: 10.1016/j.immuni.2012.01.009

Figure 4. Salmonella type III secretion protein and invasion factor SipB induces mitochondrial depolarization and caspase-1 dependent IL-1β secretion.

Figure 4

(A) Three hour-LPS-primed Nlrp3–/– BMDM were exposed to wild-type (St wt) or nonflagellated mutant St (St ΔfljB/fliC) (MOI=5) and IL-1β was quantified in culture supernatants by ELISA.

(B and C) Three hour-LPS-primed BMDM were exposed to various strains of St (MOI=5) and (B) cell viability was determined by LDH release assay or (C) ΔΨm was measured by TMRM incorporation.

(D and E) IL-1β or TNF-α were quantified in culture supernatants by ELISA in (D) WT and Casp1–/– macrophages or in (E) WT and Bcl-2 overexpressing macrophages that were exposed to St (MOI=5).

Data shown are representative of three or more independent experiments (means ± SD).* p<0.05, ** p< 0.01, *** p<0.001.