Table 1.
freshly drawn cells% | 24 h stimulated% | 48 h stimulated% | 72 h stimulated% | |
---|---|---|---|---|
CC patients (n = 22) | ||||
CD3 + CD4 + CD28+ | 70.2 ± 19.1**,a | 56.1 ± 15.2*,a,b | 70.8 ± 14.6*,b | 68.2 ± 15.2* |
CD3 + CD8 + CD28+ | 21.2 ± 13.3†† | 23.4 ± 9.8† | 26.3 ± 9.1† | 25.1 ± 12.9†† |
CD3 + sCTLA-4+ | 15.2 ± 3.2‡‡,a | 25.1 ± 4.1‡‡,a | 24.0 ± 3.9‡‡,c | 17.6 ± 2.7‡,c |
CD3 + cCTLA-4+ | 11.2 ± 1.9#,a | 32.3 ± 6.4##,a,b | 44.1 ± 5.1##,b | 47.2 ± 4.7## |
Healthy controls (n = 17) | ||||
CD3 + CD4 + CD28+ | 93.4 ± 8.2**,a | 70.0 ± 7.9*,a,b | 89.2 ± 6.8*,b | 87.3 ± 8.1* |
CD3 + CD8 + CD28+ | 70.2 ± 6.9††,a | 53.1 ± 4.5†,a,b | 66.2 ± 6.1†,b | 69.3 ± 5.4†† |
CD3 + sCTLA-4+ | 1.9 ± 0.5‡‡,a | 3.5 ± 0.9‡‡,a,b | 4.8 ± 1.1‡‡,b,c | 7.9 ± 1.9‡,c |
CD3 + cCTLA-4+ | 1.4 ± 0.2#,a | 2.9 ± 0.8##,a | 3.5 ± 1.1##,c | 6.2 ± 1.5##,c |
*Comparison between CC patients and healthy controls. One symbol indicates p < 0.05; two symbols p < 0.04.
†Comparison between CC patients and healthy controls. One symbol indicates p < 0.01; two symbols p < 0.001.
‡Comparison between CC patients and healthy controls. One symbol indicates p < 0.01; two symbols p < 0.00001.
#Comparison between CC patients and healthy controls. One symbol indicates p < 0.0001; two symbols p < 0.00001.
aComparison between freshly drawn cells and 24 h stimulated cells; p < 0.04.
bComparison between 24 h stimulated cells and 48 h stimulated cells; p < 0.05.
cComparison between 48 h stimulated cells and 72 h stimulated cells; p < 0.02.
sCTLA-4 – surface CTLA-4; cCTLA-4 – cytoplasmic CTLA-4