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(A) Steps in the strategy for the attachment of R moiety in reagent II to rhodopsin cysteine mutants bound to 1D4-Sepharose beads, complex formation with GDP-Tαβγ on illumination, crosslinking by increasing pH to 7.5 or higher. (B) Steps in the identification of crosslinking site, carrying the HS-CH2-CH2-CO-NH group to a peptide sequence in Tα.
