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(A) MALDI-TOF mass spectrum of peptide fragments containing MBB-β-thio-propionyloxyamido-Tα obtained after purification on avidin-agarose of trypsin-digested peptide mixture. (B) Mass spectrum of a control sample in which trypsin alone was subjected to the trypsin digestion conditions used in A. Mass (m/z) lines (blue) originate from trypsin autolysis whereas the mass (m/z) values (red) originated crosslinked Tα.
