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Solution 19F NMR spectra in the dark of WT and the two dicysteine mutants. The cysteine residues were derivatized with the TET group as described in the text. All samples contained 0.2 mM TFA as an internal reference, which was set to 0.0 ppm. Approximately 2,000 scans were averaged per spectrum. (A) WT Cys-140–Cys-316. (B) Cys-65–Cys-316. (C) Cys-139–Cys-251.
