Table 1.
Mutant | Line, ppm* | T1, sec† |
---|---|---|
WT | 10.6 | 0.34 ± 0.01 |
WT | 10.0 | 0.39 ± 0.01 |
Cys-65–Cys-316 | 9.7 | 0.31 ± 0.02 |
Cys-65–Cys-316 | 9.2 | 0.30 ± 0.02 |
Cys-139–Cys-251 | 9.8 | 0.28 ± 0.03 |
Cys-139–Cys-251 | 11.1 | 0.38 ± 0.02 |
Chemical shifts are relative to TFA at 0.0 ppm.
T1 values and errors were determined by using vnmr 6.1 software.