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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Eur J Lipid Sci Technol. 2011 Nov;113(11):1321–1331. doi: 10.1002/ejlt.201100030

Figure 2. (A) Effect of FFAs on PMPMEase activity.

Figure 2

Purified PMPMEase (5 μg) was incubated with RD-PNB in the presence of 1 mM saturated FFAs or unsaturated FFAs and prostaglandins as indicated. The reactions were then analyzed by HPLC. The residual enzymatic activities are expressed as the mean relative to the controls (± SEM, N=3). *P<0.05, **P<0.01 and ***P<0.001 versus untreated controls compared by ANOVA, followed by the Dunnett’s post-test. (B) Inhibition of PMPMEase by FFAs and PGs. Purified PMPMEase (5 μg) was incubated with RD-PNB in the presence of varying concentrations of the indicated FFAs or PGs for 1 h. The reactions were stopped with methanol and analyzed for the residual PMPMEase activity as described in the methods section. The results are expressed as the means (± SEM, N=3) relative to the controls.