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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Eur J Lipid Sci Technol. 2011 Nov;113(11):1321–1331. doi: 10.1002/ejlt.201100030

Figure 3. Inhibition of PMPMEase activity by FFAs.

Figure 3

PMPMEase (5 μg) was incubated with RD-PNB substrate (1 mM) in the presence of AA (•), EPA (▲) or control (○) at 37 °C for the indicated times. The reactions were stopped with methanol, processed and the product (P) was separated from the residual substrate (S) by HPLC as described in the methods. The results are expressed as the means (± SEM, N=3) relative to the controls. *P<0.05, **P<0.01 and ***P<0.001 versus untreated controls compared by paired t-test.