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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Eur J Lipid Sci Technol. 2011 Nov;113(11):1321–1331. doi: 10.1002/ejlt.201100030

Figure 6.

Figure 6

(A): PMPMEase activity in degenerating AA-treated human neuroblastoma SH-SY5Y cells. Human neuroblastoma cells were cultured and seeded in 24-well plates as described in the methods. They were then treated with the indicated concentrations of AA or EPA. After 24 h, the cells were lysed as described in the methods and the PMPMEase activity determined using RD-PNB as the substrate. (B): AA-treated, degenerating human neuroblastoma cells were lysed and aliquots were pre-incubated with either COX-1 (23.5 units) or COX-2 (9 units) for 1 h. The RD-PNB substrate was added and the mixture further incubated for 3 h to determine the PMPMEase activity. The results are relative to the controls and are the means (± SEM, N=4). ***P<0.001 versus untreated control cells compared by ANOVA, followed by the Dunnett’s post-test.