Table 1.

Basal and Stimulated Activities of Wild-Type (WT) and HNOX Mutants Transfected in COS-7 Cells^a

	basal		PPIX, 100 μM		YC-1, 100 μM		SNAP 100 μM		SNAP, 100 μM + YC-1, 100 μM
	spec act	% of WT	spec act	fold stimul	spec act	fold stimul	spec act	fold stimul	spec act	fold stimul
WT	229 ± 7	100	431 ± 42	1.9	1430 ± 63	6	3328 ± 102	14.5	3402 ± 370	15
D102A	161 ± 21	70	160 ± 5	1	232 ± 26	1.4	354 ± 38	2.2	662 ± 129	4
D102N	47 ± 6	20.5	87 ± 14	1.85	202 ± 28	4	229 ± 17	4.9	1063 ± 77	22
D102E	73 ± 17	32	168 ± 20	2.4	128 ± 13	1.7	152 ± 82	2	292 ± 78	4
F120A	169 ± 53	73	108 ± 44	0.64	172 ± 22	1	278 ± 113	1.6	231 ± 28	1.4

^aSpecific activity (spec act) is expressed in pmol min⁻¹ mg⁻¹ of extracted protein ± SEM and is the result of three independent transfections; for each transfection, the assay was repeated three times (and in duplicate) under each condition. Fold stimulation (fold stimul.) strongly lower or higher than WT is underlined and in bold, respectively. The NO-stimulated values with SNAP were confirmed using DEA-NO (not shown). DEA-NO was then used with purified sGC.