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. Author manuscript; available in PMC: 2012 Mar 27.
Published in final edited form as: Cancer Biol Ther. 2009 Jun 16;8(12):1136–1145. doi: 10.4161/cbt.8.12.8446

Figure 4.

Figure 4

Low HU treatment decreases TRF2-DNA binding activity. (A) HCT116 cells were transfected with a FLAG-TRF2 plasmid and treated with low HU for one day. Western blots of the inputs of the transfection are shown to the left and the FLAG-eluted protein is shown the right. (B) Increasing concentrations of FLAG-TRF2 protein (1, 5 and 9 μl) were mixed with a radio-labeled duplex telomere probe and electrophoresed on a 6.0% polyacrylamide gel and binding was assessed via PhosphoImage analysis of the dried gel. (C) PhosphorImager quantification of the percent of (TTAGGG)6 probe bound by TRF2 without or with HU treatment from three independent EMSA assays. Untreated TRF2 binding was normalized to 1% for each experiment. Errror bars represent standard deviation from the mean.