Figure 3.

Chronic hyperglycemia persistently upregulates TXNIP expression in rMC1 and induces IL-1β and NLRP3 inflammasome activation. rMC1 cells were cultured under HG for (a and b) 0–24 h or (c and d) 0–5 days and TXNIP proteins were detected by Western blotting. For this, cell extracts were prepared in RIPA buffer and 30 μg protein was analyzed on 12% SDS-PAGE and Western blot for cytosolic TXNIP, Pro-IL-1β, NLRP3, and pro-caspase-1 and the nuclear level of phosphorylated p65 at serine residue 276 (S276) of NF-κB. ECL detected the immunoreactive bands. Actin and tubulin were used as controls for protein loading. A representative blot for each protein is shown here from n = 3-4.