Figure 1.
Spatial and temporal expression of RDHB. A, Schematic diagram of a cross-sectional view of a single ommatidium from a Drosophila compound eye. B–D, Immunostaining of a 0.5 μm head section from an rdhB-GAL4;rdhB1, UAS-rdhB::myc fly. B, Myc antibodies (red). C, Rhodopsin (Rh1) antibodies (green). D, Merged image. E, Developmental Western blots probed with antibodies to RDHB, Rh1, and tubulin (Tub). Samples were prepared from flies at the indicated pupal times and from adult flies of the indicated ages. F, G, Immunostaining of dissected pupae eyes (∼45 h after puparium formation) with rabbit anti-RDHB. F, wild-type. The arrows indicate labeling of tertiary pigment cells. G, rdhB1.