Table 1.

Sequences of Primers and Probes Used for Real-Time PCR; Sequences Related to Gene Type X Collagen are Proprietary to Applied Biosystems Inc. and Were Not Disclosed

Gene	Forward primer	Reverse primer	Probe
GAPDH	AGGGCTGCTTTTAACTCTGGTAAA	GAATTTGCCATGGGTGGAAT	CCTCAACTACATGGTTTAC
COL I	AGGACAAGAGGCATGTCTGGTT	GGACATCAGGCGCAGGAA	TTCCAGTTCGAGTATGGC
COL II	GGCAATAGCAGGTTCACGTACA	CGATAACAGTCTTGCCCCACTT	CTGCACGAAACATAC
Aggrecan	TCGAGGACAGCGAGGCC	TCGAGGGTGTAGCGTGTAGAGA	ATGGAACACGATGCCTTTCACCACGA

PCR, polymerase chain reaction, GAPDH, glyceraldehyde-3-phosphate dehydrogenase; COL, collagen.