Table 2.
Young's Modulus (EY), Dynamic Modulus (1Hz), Glycosaminoglycan (GAG) and Collagen Contents Normalized to Wet Weight (w.w.); and GAG and Collagen Contents Normalized to DNA (ng/ng); and Construct Volume Normalized to Initial Volume on Day 0; 20/60FS: Constructs Seeded with 20 or 60 Million Cells/ml and Cultured Under Free Swelling (FS) Conditions; 20/60DL: Constructs Seeded with 20 or 60 Million Cells/ml and Cultured Under Dynamic Loading (DL) Conditions
| 20FS | 20DL | 60FS | 60DL | ||
|---|---|---|---|---|---|
| EY (kPa) | Day 30 | 6.3±0.7 | 11.8±3.3 | 12.4±3.8 | 32.0±5.3*† |
| Day 70 | 8.2±3.0 | 28.3±7.8* | 55.5±8.3† | 59.2±8.5† | |
| Dynamic modulus (kPa) | Day 30 | 190±28 | 218±14 | 329±93 | 476±56*† |
| Day 70 | 225±22 | 467±62* | 671±87† | 758±106† | |
| GAG/w.w. | Day 30 | 0.8±0.1% | 0.8±0.1% | 1.1±0.2% | 1.5±0.1%*† |
| Day 70 | 0.7±0.2% | 1.2±0.1%* | 2.3±0.4%† | 2.1±0.2%† | |
| Collagen/w.w. | Day 30 | 0.3±0.0% | 0.3±0.0% | 0.5±0.1% | 0.9±0.2%*† |
| Day 70 | 0.8±0.2% | 1.3±0.2%* | 1.7±0.2%† | 1.6±0.2%† | |
| GAG/DNA | Day 30 | 72.2±6.2 | 76.5±12.8 | 31.7±10.1† | 53.0±5.0* |
| Day 70 | 62.3±19.9 | 105.7±4.9* | 79.5±18.6 | 89.9±14.3 | |
| Collagen/DNA | Day 30 | 28.0±3.8 | 31.3±4.6 | 15.7±2.7† | 30.1±7.9* |
| Day 70 | 90.8±11.8 | 109.5±18.8 | 58.3±5.9 | 67.7±10.0† | |
| Normalized volume | Day 30 | 130.5±2.3% | 124.9±7.5% | 115.0±7.1%† | 119.6±5.7% |
| Day 70 | 118.9±4.2%‡ | 108.6±1.1%*‡ | 109.4±8.7% | 114.8±11.5% |
*
p<0.05 vs. FS group at the same culture time (n=4), †p<0.05 vs. 20FS/DL group at the same culture time (n=4), ‡p<0.05 vs. 20FS/DL group on Day 30 (n=4). Data presented as mean±standard deviation.