Table 1.
Primer Sequences and Conditions for Qualitative Real-Time Polymerase Chain Reaction
| Gene | Gene name | Primer nucleotide sequence | Product size (bp) | Annealing temperature, °C | Accession number |
|---|---|---|---|---|---|
| β-actin | β-actin | 5′-ATCGTGGGCCGCCCTAGGCA-3′ (Forward) 5′-TGGCCTTAGGGTTCAGAGGGG-3′ (Reverse) |
243 | 52 | NM_031144.2 |
| Tenomodulin | Tnmd | 5′-GTGGTCCCACAAGTGAAGGT-3′ (Forward) 5′-GTCTTCCTCGCTTGCTTGTC-3′ (Reverse) |
60 | 52 | NM_022290.1 |
| Scleraxis | Scx | 5′-AACACGGCCTTCACTGCGCTG-3′ (Forward) 5′-CAGTAGCACGTTGCCCAGGTG-3′ (Reverse) |
102 | 58 | NM_001130508.1 |
| Collagen type 1 α 1 | Col1A1 | 5′-CATCGGTGGTACTAAC-3′ (Forward) 5′-CTGGATCATATTGCACA-3′ (Reverse) |
238 | 50 | NM_053356.1 |
| Collagen type 3 α 1 | Col3A1 | 5′-GATGGCTGCACTAAAC-3′ (Forward) 5′-CGAGATTAAAGCAAGAG-3′ (Reverse) |
225 | 50 | NM_032085.1 |
| Decorin | Dcn | 5′-ATGATTGTCATAGAACTGGGC-3′ (Forward) 5′-TTGTTGTTATGAAGGTAGAC-3′ (Reverse) |
382 | 55 | NM_024129.1 |
| Tenascin C | Tnc | 5′-CAGAAGCTGAACCGGAAGTTG-3′ (Forward) 5′-GGCTGTTGTTGCTATGGCACT-3′ (Reverse) |
278 | 55 | NM_053861.1 |
| Alkaline phosphatase | Alpl | 5′-TCCGTGGGTCGGATTCCT-3′ (Forward) 5′-GCCGGCCCAAGAGAGAA-3′ (Reverse) |
85 | 58 | NM_013059.1 |
| Collagen type 2 α 1 | Col2A1 | 5′-CCGGACTGTGAGGTTAGGAT-3′ (Forward) 5′-AACCCAAAGGACCCAAATAC-3′ (Reverse) |
101 | 58 | NM_012929.1 |
| Aggrecan | Acan | 5′-CTTGGGCAGAAGAAAGATCG-3′ (Forward) 5′-GTGCTTGTAGGTGTTGGGGT-3′ (Reverse) |
159 | 58 | NM_022190.1 |
| Biglycan | Bgn | 5′-TCTACATCTCCAAGAACCACCTGG-3′ (Forward) 5′-TTGGTGATGTTGTTGGAGTGCAGA-3′ (Reverse) |
513 | 55 | NM_017087.1 |
| Sox9 | Sox9 | 5′-AGAGCGTTGCTCGGAACTGT-3′ (Forward) 5′-TCCTGGACCGAAACTGGTAAA-3′ (Reverse) |
67 | 60 | XM_343981.2 |
| Runt-related transcription factor 2 | Runx2 | 5′-CCGATGGGACCGTGGTT-3′ (Forward) 5′-CAGCAGAGGCATTTCGTAGCT-3′ (Reverse) |
74 | 60 | NM_053470.1 |
| Bone morphogenetic protein-2 | Bmp2 | 5′-TAGTGACTTTTGGCCACGACG-3′ (Forward) 5′-GCTTCCGCTGTTTGTGTTTG-3′ (Reverse) |
81 | 58 | NM_017178.1 |
| Osteopontin | Spp1 | 5′-TCCAAGGAGTATAAGCAGCGGGCCA-3′ (Forward) 5′-CTCTTAGGGTCTAGGACTAGCTTCT-3′ (Reverse) |
199 | 55 | NM_012881.2 |
| Osteocalcin | Bglap | 5′-GAGCTGCCCTGCACTGGGTG-3′ (Forward) 5′-TGGCCCCAGACCTCTTCCCG-3′ (Reverse) |
263 | 60 | M23637.1 |
| CCAAT enhancer binding protein alpha | C/EBPα | 5′-AAGGCCAAGAAGTCGGTGGA-3′ (Forward) 5′-CAGTTCGCGGCTCAGCTGTT-3′ (Reverse) |
189 | 55 | NM_012524.2 |
| Peroxisome proliferator-activated receptor gamma 2 | PPARγ2 | 5′-CGGCGATCTTGACAGGAAAG-3′ (Forward) 5′-GCTTCCACGGATCGAAACTG-3′ (Reverse) |
174 | 59 | AB019561 |
| Oct4 | Oct4 | 5′-CATCTGCCGCTTCGAG-3′ (Forward) 5′-CTCAATGCTAGTCCGCTTTC-3′ (Reverse) |
166 | 55 | NM_001009178.2 |
| Nanog | Nanog | 5′-GCCCTGATTCTTCTAGCAAT-3′ (Forward) 5′-AGAACACAGTCCGCATCTT-3′ (Reverse) |
120 | 55 | NM_001100781.1 |
| Sox2 | Sox2 | 5′-CCCACCTACAGCATGTCCTA-3′ (Forward) 5′-TGGAGTGGGAGGAAGAGGTA-3′ (Reverse) |
124 | 60 | NM_001109181.1 |
| GAPDH | Gapdh | 5′-GGTCGGTGTGAACGGATTTGG-3′ (Forward) 5′-GCCGTGGGTAGAGTCATACTGGAAC-3′ (Reverse) |
148 | 55 | NM_017008.3 |