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. Author manuscript; available in PMC: 2012 Dec 8.
Published in final edited form as: Neuron. 2011 Dec 8;72(5):760–775. doi: 10.1016/j.neuron.2011.09.031

Figure 5. Synaptic Transmission Deficiencies in ank2 Mutants Are Suppressed by Both eiger Mutations and wengen-RNAi.

Figure 5

(A) Average mEPSP amplitude for wild-type, egr, ank2, and the double mutant egr; ank2. In (A)–(D), values are averages from individual recordings, and genotypic averages are shown as large horizontal bars with SEM indicated by the position of smaller horizontal bars above and below the mean. wt = w1118 (average mEPSP, 1.23 mV, n = 26); egr = eigerΔ25/eiger Δ25 (average mEPSP, 1.2 mV, n = 24); ank2 = ank22001/ank22001 (average mEPSP, 0.98 mV, n = 40); egr; ank2 = eiger Δ25/eiger Δ25; ank22001/ank22001 (average mEPSP, 1.13 mV, n = 28). There is no significant difference between genotypes.

(B) The ank2 mutants have a large number of recordings with average EPSPs below 25 mV, the limit of the wild-type distribution (red dash line). wt = w1118 (average EPSP, 44.2 mV, n = 26); egr = egrΔ25/egrΔ25 (average EPSP, 42.8mV, n = 24); ank2 = ank22001/ank22001 (average EPSP, 29.5mV, n = 40); egr; ank2 = egrΔ25/egrΔ25; ank22001/ank22001 (average EPSP, 35.9 mV, n = 28). A significant increase in EPSP amplitude is observed comparing ank2 with egr; ank2 (*p = 0.02, Student’s t test).

(C) Average mEPSP amplitude for wild-type, wgn-RNAi, ank2, and wgn-RNAi; ank2 larva. wt = w1118 (average mEPSP, 1.23 mV, n = 26); wgnRNAi = elavC155-GAL4; wgn-RNAi (average mEPSP, 1.41 mV, n = 16); ank2 = ank22001/ank22001 (average mEPSP, 0.98 mV, n = 40); wgnRNAi; ank2 = elavC155-GAL4; wgn-RNAi/+; ank2/ank2 (average mEPSP, 0.96 mV, n = 30). There is no difference comparing ank2 with wgn-RNAi/+; ank2/ank2. n.s., not significant.

(D) Average EPSP amplitude for wild-type, wgn-RNAi, ank2, and wgn-RNAi; ank2 larva. wt = w1118 (average EPSP, 44.2 mV, n = 26); wgnRNAi = elavC155-GAL4; wgn-RNAi (average EPSP, 48.08 mV, n = 16); ank2 = ank22001/ank22001 (average EPSP, 29.5 mV, n = 40); wgnRNAi; ank2 = elavC155-GAL4; wgn-RNAi/+; ank2/ank2 (average EPSP, 32.8 mV, n = 30). There is a significant increase in average EPSP amplitude comparing ank2 and wgnRNAi; ank2 (*p = 0.05, Student’s t test).

(E) Representative EPSP and mEPSP traces for wild-type, eiger, ank2, and egr; ank2.