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. Author manuscript; available in PMC: 2013 Feb 6.
Published in final edited form as: Mol Pharm. 2012 Jan 20;9(2):325–333. doi: 10.1021/mp2005577

Figure 4.

Figure 4

Co(III)-Ci is unable to inhibit the Krüppel zinc finger domain from binding DNA. (A) Lane 1. 10 nM of a 32P radiolabeled Kr probe. The Kr zinc finger domain was overexpressed in S2 cells, cells were lysed and incubated for 3 hours with buffer (lane 2), 1X or 50X Co(III)-Ci (lanes 3 and 4), 1X or 50X Ci Cold Oligos (lanes 5 and 6) or 1X or 50X Kr Cold Oligos (lanes 7 and 8). Then 10 nM of a 32P radiolabeled Kr probe was added to the reactions for 15 minutes and the reactions were analyzed by EMSA as previously described. Reactions were performed in triplicate (representative gel is shown). The relevant Kr specific shift is indicated by an arrow and (B) was quantified using ImageQuant software. The higher, non-specific band (see Supporting Information Fig. 6) was left out of the analysis. Error bars are one standard deviation. Final reaction concentrations are given.