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. Author manuscript; available in PMC: 2012 Mar 27.
Published in final edited form as: J Immunol. 2010 May 7;184(11):6514–6521. doi: 10.4049/jimmunol.0900448

Figure 1. Native and superagonist CTL determinants can be distinguised in bead-based cross-presentation assay.

Figure 1

Oligonucleotides encoding MART-126-35, NY-ESO-1157-165, or MART-126-35A27L were cloned into and expressed by pQE40 expression vectors in 5ml bacterial cultures. The mini-gene products were isolated and “fed” to immature dendritic cells as described in materials and methods. MART-126-35-specific CTL clones were used to detect the presence of the cross-presented mini-gene products. Induced IFN-γ expression was determined by standard sandwich ELISA. A27L synthetic peptide at 1μM was used a positive control. Experiment was performed in triplicate.