FIG. 3.

TUNEL analyses of Sertoli cell and MSC-1 cell grafts are shown. Graft-bearing kidneys were removed 8 days after transplantation of apSC (A, B) or aMSC-1 (C, D), and tissue sections were analyzed for apoptotic cells by TUNEL assay (green color [A, C]). Sections were counterstained with bisBenzimide (Hoechst 33342; blue color [B, D]). A dotted line separates grafts from kidney (K). Insets in A and C show superimposed images of TUNEL-positive cells among Hoechst dye-positive cells at a higher magnification. E) Relative fold changes in the number of apoptotic cells in the SC (grey bar) or MSC-1 cell (black bar) grafts are shown. Data shown are means ± SEM for at least three different experiments per time point. Different letters denote significant differences in means determined by ANOVA followed by the Student t-test method at a P value of ≤0.05.