FIG. 5.

Real-time RT-PCR results show normalized signal expression and fold difference between known Sertoli cells genes in aMSC1 and those in apSC. The y axis shows the average normalized means ± SEM of transcript level for aMSC1 (white bar) and apSC (black bar) normalized to Rps2 for Lrat, Inha, Fshr, Star, and Clu (A), and Crem (B) transcript levels. Real-time RT-PCR was conducted in triplicate using three biological samples per cell type. Relative fold differences in apSC compared to those in aMSC1 are designated above each gene. *, significant differences at P ≤ 0.01.