SLC26A3, SLC26A6, and SLC9A3R1 colocalize with CFTR. Swim-up-separated sperm were fixed, permeabilized, and exposed to anti-SLC26A3 (A), anti-SLC26A6 (B), and anti-SLC9A3R1 (C) (red signal); the same cells were also exposed to anti-CFTR (green or blue signal). The merged and phase-contrast images are also shown (DIC). Bar = 20 μm. Images are representative of four independent experiments.